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Digital Holographic Microscopy for Phenotypic Profiling of Adherent Cells.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2026

Lam Van K, Bazow Brad, Phan Thuc, Nehmetallah George, Raub Christopher B

Abstract excerpt

Digital holographic microscopy and other quantitative phase imaging methods provide accurate reconstructions of the microscale features of cells, related to cell shape, height, and index of refraction, useful for phenotypic profiling. The following section details a typical cell imaging experiment by digital holographic microscopy, image analysis, and machine learning steps directed toward phenotypic profiling of...

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PMID: 41479053
DOI: 10.1007/978-1-0716-4985-5_9

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