Article
CRISPR/Cas12a-SERS biosensor based on sea urchin-like AuNPs for the detection of β-thalassemia mutant gene CD31.
Mikrochimica acta - 14 Nov 2025
Xu Huaqiu, Chen Huagan, Li Yixing, Wang Yue, Zeng Huihui, Xu Ziyu, Chen Tingsha, Kong Delan, Huang Wenyi, Cheng Hao, Zhou Haibo, Jiang Xudong, Feng Jun
Abstract excerpt
Beta-thalassemia is a single-gene recessive disorder caused by mutations in the HBB gene, and approximately 1.5% of the global population are carriers of β-thalassemia. It is therefore vital to establish a rapid and sensitive method to detect the mutant genes of β-thalassemia. In this study, a CRISPR/Cas12a-mediated amplification-free surface-enhanced Raman spectroscopy (SERS) biosensor was developed. This...
Topics
- Biosensing Techniques
- beta-Thalassemia
- CRISPR-Cas Systems
- Animals
- Spectrum Analysis, Raman
- Gold
- Metal Nanoparticles
- Mutation
- Sea Urchins
- Limit of Detection