Article
Multiplex Real-Time PCR Assay Using TaqMan Probes for the Identification of Trypanosoma cruzi DTUs in Biological and Clinical Samples.
PLoS neglected tropical diseases - 1 May 2015
Cura Carolina I, Duffy Tomas, Lucero Raúl H, Bisio Margarita, Péneau Julie, Jimenez-Coello Matilde, Calabuig Eva, Gimenez María J, Valencia Ayala Edward, Kjos Sonia A, Santalla José, Mahaney Susan M, Cayo Nelly M, Nagel Claudia, Barcán Laura, Málaga Machaca Edith S, Acosta Viana Karla Y, Brutus Laurent, Ocampo Susana B, Aznar Christine, Cuba Cuba Cesar A, Gürtler Ricardo E, Ramsey Janine M, Ribeiro Isabela, VandeBerg John L, Yadon Zaida E, Osuna Antonio, Schijman Alejandro G
Abstract excerpt
BACKGROUND: Trypanosoma cruzi has been classified into six Discrete Typing Units (DTUs), designated as TcI-TcVI. In order to effectively use this standardized nomenclature, a reproducible genotyping strategy is imperative. Several typing schemes have been developed with variable levels of complexity, selectivity and analytical sensitivity. Most of them can be only applied to cultured stocks. In this context, we...
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