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An Optimized, CE-Compatible, Targeted NGS-Based SSR Genotyping Method Using Primer-Anchored Alignment.

Electrophoresis - 1 Jun 2025

Liu Kai, Meng Qinghui, Zheng Tianlun, Xie Nan, Zhou Fan

Abstract excerpt

Microsatellites (SSRs) are highly polymorphic DNA sequences widely used in genetic research, including parentage assignment. Traditional SSR analysis relies on capillary electrophoresis (CE), which is time-consuming and has limited capacity. Next-generation sequencing (NGS) offers a high-throughput and cost-effective alternative, but existing NGS-based SSR genotyping methods produce results that are incompatible...

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PMID: 40614165
DOI: 10.1002/elps.70007

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