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Improving the solubility of single domain antibodies using VH ‐like hallmark residues

16 Jun 2025

Abstract excerpt

Single domain antibodies (sdAbs) can be generated from variable regions of heavy-chain antibodies, which lack light chain and CH1 region. They have attracted attention due to their small size and molecular characteristics. Hydrophilic hallmark amino acids at framework region 2 (FR2) are key residues involved in the solubility of sdAbs. Nevertheless, previous studies reported that several sdAbs with human VH-like...

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PMID: 40521627
DOI: 10.1002/pro.70189

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