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A one-pot method for universal Dengue virus detection by combining RT-RPA amplification and CRISPR/Cas12a assay

24 Mar 2025

Abstract excerpt

Dengue Virus (DENV) is a life-threatening pathogen leading to dengue fever, which brings about huge public health challenges globally. However, traditional detection methods currently fail to meet the increasing demands of clinic practice in terms of speed, simplicity, and accuracy. To address these limitations, we developed a novel, rapid, and highly sensitive diagnostic method for universal DENV detection by...

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PMID: 40128655
DOI: 10.1186/s12866-025-03882-z

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