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Detection of monkeypox virus based on a convenient and sensitive single-step RPA-CRISPR/Cas12a strategy

1 Jan 2024

Abstract excerpt

The global outbreak of monkeypox virus (MPXV) has highlighted the need for rapid molecular diagnostics techniques. In this study, a single-step recombinase polymerase amplification (RPA)-CRISPR/Cas12a system was developed for rapid and sensitive detection of MPXV. The limit of detection of this assay was 1 copy per μL of extracted nucleic acids. A heating lysis method was integrated to further simplify the sample...

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PMID: 38716102
DOI: 10.1039/d4ra02049a

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