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High-efficient molecular detection system termed RAA-based CRISPR-Cas13a for novel duck orthoreovirus

29 May 2025

Abstract excerpt

The novel duck orthoreovirus (NDRV) is an immunosuppressive pathogen that significantly impacts the health of waterfowl breeding. Accurate, efficient, and convenient detection techniques are crucial for the prevention and control of NDRV, particularly in terms of field detection. By employing recombinase aided amplification (RAA) and clustered regularly interspaced short palindromic repeats (CRISPR)-Cas13a, we...

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PMID: 40466269
DOI: 10.1016/j.psj.2025.105327

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