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Rapid detection of Nipah virus using the one-pot RPA-CRISPR/Cas13a assay

15 May 2023

Abstract excerpt

Nipah virus (NiV) is a zoonotic pathogen with airborne transmission and high case fatality rates in humans. There is currently no treatment or vaccine against NiV infection approved for humans or animals, therefore early diagnosis is the key to control any potential outbreaks. In this study, we developed an optimized one-pot assay using recombinase polymerase amplification (RPA) coupled to CRISPR/Cas13a for the...

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PMID: 37178792
DOI: 10.1016/j.virusres.2023.199130

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