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Article

Molecular basis for thiocarboxylation and release of Urm1 by its E1-activating enzyme Uba4.

Nucleic acids research - 11 Dec 2024

Sokołowski Mikołaj, Kwasna Dominika, Ravichandran Keerthiraju E, Eggers Cristian, Krutyhołowa Rościsław, Kaczmarczyk Magdalena, Skupien-Rabian Bozena, Jaciuk Marcin, Walczak Marta, Dahate Priyanka, Pabis Marta, Jemioła-Rzemińska Małgorzata, Jankowska Urszula, Leidel Sebastian A, Glatt Sebastian

Abstract excerpt

Ubiquitin-related modifier 1 (Urm1) is a highly conserved member of the ubiquitin-like (UBL) family of proteins. Urm1 is a key component of the eukaryotic transfer RNA (tRNA) thiolation cascade, responsible for introducing sulfur at wobble uridine (U34) in several eukaryotic tRNAs. Urm1 must be thiocarboxylated (Urm1-SH) by its E1 activating enzyme UBL protein activator 4 (Uba4). Uba4 first adenylates and then...

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Cryoelectron Microscopy
Nucleotidyltransferases
Protein Domains
RNA, Transfer

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Saccharomyces cerevisiae Proteins

Sulfhydryl Compounds

Ubiquitin-Activating Enzymes

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Identifiers

PMID: 39673271
DOI: 10.1093/nar/gkae1111
PMCID: PMC11662940

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RHD

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 1 Sept 2026.

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