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Host DNA depletion on frozen human respiratory samples enables successful metagenomic sequencing for microbiome studies

28 Nov 2024

Abstract excerpt

Most respiratory microbiome studies use amplicon sequencing due to high host DNA. Metagenomics sequencing offers finer taxonomic resolution, phage assessment, and functional characterization. We evaluated five host DNA depletion methods on frozen nasal swabs from healthy adults, sputum from people with cystic fibrosis (pwCF), and bronchoalveolar lavage (BAL) from critically ill patients. Median sequencing depth...

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PMID: 39609616
DOI: 10.1038/s42003-024-07290-3

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