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Article

Harnessing Oxidative Stress to Obtain Natural Riboflavin Secreting Lactic Acid Bacteria for Use in Biofortification.

Journal of agricultural and food chemistry - 27 Nov 2024

Rasmussen Emmelie Joe Freudenberg, Acs Norbert, Jensen Peter Ruhdal, Solem Christian

Abstract excerpt

Lactococcus lactis suffers from oxidative stress and riboflavin starvation at elevated temperatures due to dissolved oxygen, which can be relieved partially by exogenously supplied riboflavin. Here we explore whether this phenomenon can be harnessed to obtain riboflavin overproducing mutants. Using a riboflavin auxotrophic L. lactis strain as a riboflavin biosensor, we screened L. lactis cultures that had been...

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PMID: 39540590
DOI: 10.1021/acs.jafc.4c08881

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