Article
New insight into acid-resistant enzymes from natural mutations of Escherichia coli Nissle 1917.
Enzyme and microbial technology - 1 Dec 2024
Xue Chengfeng, Ting Wan-Wen, Juo Jiun-Jang, Ng I-Son
Abstract excerpt
The probiotic Escherichia coli Nissle 1917 (EcN), known for its superior acid resistance (AR), serves as a promising chassis for live therapeutics due to the effective colonization capabilities. However, the enzymatic activity regarding AR in EcN remains poorly understood. First, we investigated the AR systems of EcN by measuring cell growth under acidic stress and exploring the relationship of mutations to their...
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