Article
Efficient synthesis of CRISPR-Cas13a-antimicrobial capsids against MRSA facilitated by silent mutation incorporation.
Scientific reports - 13 Jul 2024
Shimamori Yuzuki, Tan Xin-Ee, Li Feng-Yu, Nishikawa Yutaro, Watanabe Shinya, Sasahara Teppei, Miyanaga Kazuhiko, Aiba Yoshifumi, Veeranarayanan Srivani, Thitiananpakorn Kanate, Nguyen Huong Minh, Batbold Anujin, Nayanjin Tergel, Lian Adeline Yeo Syin, Hossain Sarah, Kawaguchi Tomofumi, Alessa Ola, Kumwenda Geofrey, Sarangi Jayathilake, Revilleza Jastin Edrian C, Baranwal Priyanka, Arbaah Mahmoud, Maniruzzaman, Yi Liu, Duyen Ho Thi My, Sugano Takashi, Sultana Sharmin, Faruk Mohammad Omar, Hidaka Yuya, Thu Myat, Shimojyo Takayuki, Kiga Kotaro, Cui Longzhu
Abstract excerpt
In response to the escalating global threat of antimicrobial resistance, our laboratory has established a phagemid packaging system for the generation of CRISPR-Cas13a-antimicrobial capsids targeting methicillin-resistant Staphylococcus aureus (MRSA). However, a significant challenge arose during the packaging process: the unintentional production of wild-type phages alongside the antimicrobial capsids. To...
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