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Article

Recognition of phospholipids in Streptomyces phospholipase D.

The Journal of biological chemistry - 15 Jul 2005

Uesugi Yoshiko, Mori Koichi, Arima Jiro, Iwabuchi Masaki, Hatanaka Tadashi

Abstract excerpt

To investigate the contribution of amino acid residues to the enzyme reaction of Streptomyces phospholipase D (PLD), we constructed a chimeric gene library between two highly homologous plds, which indicated different activity in transphosphatidylation, using RIBS (repeat-length independent and broad spectrum) in vivo DNA shuffling. By comparing the activities of chimeras, six candidate residues related to...

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PMID: 15899903
DOI: 10.1074/jbc.M414319200

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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