Article
Comparative quantification of human intestinal bacteria based on cPCR and LDR/LCR.
World journal of gastroenterology - 21 Jan 2012
Tang Zhou-Rui, Li Kai, Zhou Yu-Xun, Xiao Zhen-Xian, Xiao Jun-Hua, Huang Rui, Gu Guo-Hao
Abstract excerpt
AIM: To establish a multiple detection method based on comparative polymerase chain reaction (cPCR) and ligase detection reaction (LDR)/ligase chain reaction (LCR) to quantify the intestinal bacterial components. METHODS: Comparative quantification of 16S rDNAs from different intestinal bacterial components was used to quantify multiple intestinal bacteria. The 16S rDNAs of different bacteria were amplified...
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