Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Development of a Novel Long-Range 16S rRNA Universal Primer Set for Metagenomic Analysis of Gastrointestinal Microbiota in Newborn Infants

28 Jun 2014

Abstract excerpt

Metagenomic analysis of the human intestinal microbiota has extended our understanding of the role of these bacteria in improving human intestinal health; however, a number of reports have shown that current total fecal DNA extraction methods and 16S rRNA universal primer sets could affect the species coverage and resolution of these analyses. Here, we improved the extraction method for total DNA from human fecal...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Clostridium difficile and Clostridium perfringens research
Share to Gut microbiota and health
Share to Pediatric Hepatobiliary Diseases and Treatments

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Source

Identifiers

PMID: 24722376
DOI: 10.4014/jmb.1403.03032

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 2 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Development of a Novel Long-Range 16S rRNA Universal Primer Set for Metagenomic Analysis of Gastrointestinal Microbiota in Newborn InfantsPMID 24722376Read ↗

Counting the Countless: Bacterial Quantification by Targeting rRNA Molecules to Explore the Human Gut Microbiota in Health and DiseaseSemantic proximity Read ↗

Quantitative and dynamic profiling of human gut core microbiota by real-time PCRSemantic proximity Read ↗

16S rRNA gene primer choice impacts off-target amplification in human gastrointestinal tract biopsies and microbiome profilingSemantic proximity Read ↗

Development of an extensive set of 16S rDNA-targeted primers for quantification of pathogenic and indigenous bacteria in faecal samples by real-time PCRSemantic proximity Read ↗

Guided Protocol for Fecal Microbial Characterization by 16S rRNA-Amplicon SequencingSemantic proximity Read ↗

Comparative analysis of 16S rRNA gene and metagenome sequencing in pediatric gut microbiomesSemantic proximity Read ↗

Full-length 16S rRNA gene amplicon analysis of human gut microbiota using MinION™ nanopore sequencing confers species-level resolutionSemantic proximity Read ↗

Evaluation of an effective detection and quantification method for particular microorganisms by comparing NGS-based metagenome profiling dataSemantic proximity Read ↗

Comparative analysis of human gut bacterial microbiota between shallow shotgun metagenomic sequencing and full-length 16S rDNA amplicon sequencingSemantic proximity Read ↗

Full-length 16S rRNA gene amplicon analysis of human gut microbiota using MinION™ nanopore sequencing confers species-level resolutionSemantic proximity Read ↗

Evaluation of fecal DNA extraction protocols for human gut microbiome studiesSemantic proximity Read ↗

Gut Microbiota Analysis Results Are Highly Dependent on the 16S rRNA Gene Target Region, Whereas the Impact of DNA Extraction Is MinorSemantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.