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Isolation, Culture, and Phenotypic Analysis of Murine Lung Organoids.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2024

Evans Kelly, Dabrowska Catherine, Ng Minn E, Brainson Christine F, Lee Joo-Hyeon

Abstract excerpt

Three-dimensional (3D) organoid cultures retain self-renewing stem cells that differentiate into multiple cell types that display spatial organization and functional key features, providing a highly physiological relevant system. Here we describe a strategy for the generation of 3D murine lung organoids derived from freshly isolated primary tracheal and distal lung epithelial stem cells. Isolated tracheas are...

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PMID: 39008171
DOI: 10.1007/978-1-0716-3854-5_1

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