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Sensitivity analysis of RT-qPCR and RT-ddPCR for SARS-CoV-2 detection with mutations on N1 and E primer-probe region.

Microbiology spectrum - 6 Aug 2024

Liu Shiyou, Chai Xiaoru, Liu Chao, Bai Jiaxuan, Meng Juntao, Tian Hong, Han Xu, Han Guangyue, Li Qi, Xu Xiangdong

Abstract excerpt

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an RNA virus that undergoes rapid mutation. Based on viral whole genome sequencing analysis in Hebei Province, China, we identified several essential single nucleotide variants (SNVs) on primer-probe regions accumulating within some Omicron variants' genomes. In this study, we focused on three SNVs, C28290T, T28297C, and C28311T emerging on...

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Identifiers

PMID: 38916349
DOI: 10.1128/spectrum.04292-23
PMCID: PMC11302302

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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