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Cell Culture Differentiation and Proliferation Conditions Influence the In Vitro Regeneration of the Human Airway Epithelium

6 Jun 2024

Abstract excerpt

Abstract The human airway mucociliary epithelium can be recapitulated in vitro using primary cells cultured in an air–liquid interface (ALI), a reliable surrogate to perform pathophysiological studies. As tremendous variations exist among media used for ALI-cultured human airway epithelial cells, the aim of our study was to evaluate the impact of several media (BEGM, PneumaCult, Half & Half, and Clancy) on cell...

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PMID: 38843491
DOI: 10.1165/rcmb.2023-0356ma

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