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Article

Protocol for forward genetic screening to identify novel factors involved in a biological process in Caenorhabditis elegans.

STAR protocols - 21 Jun 2024

Yoshida Keita, Suehiro Yuji, Mitani Shohei

Abstract excerpt

Forward genetic screens have been a powerful tool for discovering genes involved in various biological processes in Caenorhabditis elegans. Here, we present a protocol for forward genetic screening to identify novel factors involved in a biological process in C. elegans. We describe steps for mutagenesis, screening, and backcrossing. To save time and effort, we also detail procedures for utilizing whole-genome...

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Identifiers

PMID: 38843399
DOI: 10.1016/j.xpro.2024.103114
PMCID: PMC11216056

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