Article
HPV genotyping in clinical samples using long-read single-molecule real-time sequencing.
Journal of medical virology - 1 May 2024
Pasquier Christophe, Raymond Stéphanie, Carcenac Romain, Demmou Sofia, Ranger Noémie, Nicot Florence, Izopet Jacques
Abstract excerpt
Human papillomavirus (HPV) genotyping is widely used, particularly in combination with high-risk (HR) HPV tests for cervical cancer screening. We developed a genotyping method using sequences of approximately 800 bp in the E6/E7 region obtained by PacBio single molecule real-time sequencing (SMRT) and evaluated its performance against MY09-11 L1 sequencing and after the APTIMA HPV genotyping assay. The levels of...
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