Article
Synchronizing Drosophila larvae with the salivary gland reporter Sgs3-GFP for discovery of phenotypes in the late third instar stage.
Developmental biology - 1 Aug 2024
McPherson W Kyle, Van Gorder Elizabeth E, Hilovsky Dalton L, Jamali Leila A, Keliinui Cami N, Suzawa Miyuki, Bland Michelle L
Abstract excerpt
The larval stage of the Drosophila melanogaster life cycle is characterized by rapid growth and nutrient storage that occur over three instar stages separated by molts. In the third instar, the steroid hormone ecdysone drives key developmental processes and behaviors that occur in a temporally-controlled sequence and prepare the animal to undergo metamorphosis. Accurately staging Drosophila larvae within the...
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