Article
In Situ Cas12a-Based Allele-Specific PCR for Imaging Single-Nucleotide Variations in Foodborne Pathogenic Bacteria.
Analytical chemistry - 6 Feb 2024
Liu Xinmiao, Yang Hao, Liu Jun, Liu Kerui, Jin Lulu, Zhang Yong, Khan Mohammad Rizwan, Zhong Kai, Cao Jijuan, He Qiang, Xia Xuhan, Deng Ruijie
Abstract excerpt
In situ profiling of single-nucleotide variations (SNVs) can elucidate drug-resistant genotypes with single-cell resolution. The capacity to directly "see" genetic information is crucial for investigating the relationship between mutated genes and phenotypes. Fluorescence in situ hybridization serves as a canonical tool for genetic imaging; however, it cannot detect subtle sequence alteration including SNVs....
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