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A rapid isothermal CRISPR-Cas13a diagnostic test for genital herpes simplex virus infection

27 Nov 2023

Abstract excerpt

types 1 and 2 (HSV-1/2). Existing diagnostic methods are not widely available that required expensive or additional equipment for conducting examinations and result readouts, which can limit their utility in resource-constrained settings. We successfully developed a CRISPR-Cas13a-based assay for the detection and genotyping of HSV. Our assay demonstrated a high sensitivity of 96.15% and 95.15% for HSV-1 and...

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PMID: 38213624
DOI: 10.1016/j.isci.2023.108581

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