Article
Multiplexing PCR allows the identification of within-species genetic diversity in ancient eDNA.
Molecular ecology resources - 1 Apr 2024
Lammers Y, Taberlet P, Coissac E, Elliott L D, Merkel M F, Pitelkova I, Alsos I G
Abstract excerpt
Sedimentary ancient DNA (sedaDNA) has rarely been used to obtain population-level data due to either a lack of taxonomic resolution for the molecular method used, limitations in the reference material or inefficient methods. Here, we present the potential of multiplexing different PCR primers to retrieve population-level genetic data from sedaDNA samples. Vaccinium uliginosum (Ericaceae) is a widespread species...
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