Mitochondrial genomes of Psylloidea (Hemiptera): candidate broad-range primers for long-polymerase chain reaction, genewise variability, and calibration-sensitive time-calibrated phylogeny.

Abstract excerpt

Psyllids (Hemiptera: Psylloidea) are economically important sap-feeders, yet comparable mitochondrial genome (mitogenome) resources remain unevenly developed, partly because amplification success is often lineage-dependent. We designed 6 candidate broad-range long-PCR primer pairs from alignments of 60 published psyllid mitogenomes and evaluated them using a long-PCR/Sanger workflow and independent Illumina...

Topics

Share this publication in a Topic to start or enrich a Post.

• Share to DNA Primers
• Share to Genetic Variation