Article
Targeted multiplex next-generation sequencing: advances in techniques of mitochondrial and nuclear DNA sequencing for population genomics.
Molecular ecology resources - 1 Mar 2013
Hancock-Hanser Brittany L, Frey Amy, Leslie Matthew S, Dutton Peter H, Archer Frederick I, Morin Phillip A
Abstract excerpt
Next-generation sequencing (NGS) is emerging as an efficient and cost-effective tool in population genomic analyses of nonmodel organisms, allowing simultaneous resequencing of many regions of multi-genomic DNA from multiplexed samples. Here, we detail our synthesis of protocols for targeted resequencing of mitochondrial and nuclear loci by generating indexed genomic libraries for multiplexing up to 100...
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