Article
Multiplexing with three-primer PCR for rapid and economical microsatellite validation.
Hereditas - 1 Jun 2014
Vartia Salla, Collins Patrick C, Cross Thomas F, Fitzgerald Richard D, Gauthier David T, McGinnity Philip, Mirimin Luca, Carlsson Jens
Abstract excerpt
The next generation sequencing revolution has enabled rapid discovery of genetic markers, however, development of fully functioning new markers still requires a long and costly process of marker validation. This study reports a rapid and economical approach for the validation and deployment of polymorphic microsatellite markers obtained from a 454 pyrosequencing library of Atlantic cod, Gadus morhua, Linnaeus...
Topics
- Animals
- DNA Primers
- Gadus morhua
- Genotype
- High-Throughput Nucleotide Sequencing
- Microsatellite Repeats
- Oceans and Seas
- Polymerase Chain Reaction
- Validation Studies as Topic