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Evaluation of a combined detection of SARS-CoV-2 and its variants using real-time allele-specific PCR strategy: an advantage for clinical practice.

Epidemiology and infection - 24 Nov 2023

Chaves-Blanco Lucía, de Salazar Adolfo, Fuentes Ana, Viñuela Laura, Perez-Florido Javier, Dopazo Joaquín, García Federico

Abstract excerpt

This study aimed to assess the ability of a real-time reverse transcription polymerase chain reaction (RT-PCR) with multiple targets to detect SARS-CoV-2 and its variants in a single test. Nasopharyngeal specimens were collected from patients in Granada, Spain, between January 2021 and December 2022. Five allele-specific RT-PCR kits were used sequentially, with each kit designed to detect a predominant variant at...

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Identifiers

PMID: 37997654
DOI: 10.1017/S095026882300184X
PMCID: PMC10753446

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