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Article

Rapid detection of SARS-CoV-2 variants using allele-specific PCR.

Journal of virological methods - 1 May 2022

Brito-Mutunayagam Sanjita, Maloney Daniel, McAllister Gina, Dewar Rebecca, McHugh Martin, Templeton Kate

Abstract excerpt

Tracking severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants through whole genome sequencing (WGS) is vital for effective infection control and prevention (IPC) measures, but can be time-consuming and resource-heavy. We describe an in-house validation of an allele-specific polymerase chain reaction (ASP) variant assay to detect variants of concern (VOC). ASP sensitivity for detecting Delta,...

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Identifiers

PMID: 35182711
DOI: 10.1016/j.jviromet.2022.114497
PMCID: PMC8848537

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