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Article

Protocol for isolation of signet ring cells from human gastric mucosa.

STAR protocols - 15 Dec 2023

Samaranayake Sarah G, Gamble Lauren A, Bowden Cassidy, Green Benjamin L, Gallanis Amber F, Akbulut Dilara, Shah Niharika, Hernandez Jonathan M, Davis Jeremy L

Abstract excerpt

More than 90% of individuals with germline pathogenic CDH1 variants will harbor occult, microscopic foci of signet ring cell carcinomas capable of progressing to advanced diffuse-type gastric cancer. Here, we present a protocol for high viability suspension of signet ring cells from human gastric tissue. We describe the steps for gastric mucosa isolation and tissue dissociation. We then detail procedures for...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 37925632
DOI: 10.1016/j.xpro.2023.102695
PMCID: PMC10652202

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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