Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Optimization and prospective evaluation of sensitive real-time PCR assays with an internal control for the diagnosis of melioidosis in Thailand

11 Oct 2023

Abstract excerpt

ABSTRACT Melioidosis is a severe infectious disease caused by Burkholderia pseudomallei . Effective treatment of melioidosis requires rapid detection of B. pseudomallei infection. Previous studies demonstrated that real-time PCR for the diagnosis of melioidosis in blood samples was insensitive. We optimized the DNA extraction method and evaluated real-time PCRs with an internal control for the detection of B....

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Brucella: diagnosis, epidemiology, treatment
Share to Burkholderia infections and melioidosis

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Source

Identifiers

PMID: 37819125
DOI: 10.1128/spectrum.01039-23

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Optimization and prospective evaluation of sensitive real-time PCR assays with an internal control for the diagnosis of melioidosis in ThailandPMID 37819125Read ↗

A Quadruplex Real-Time PCR Assay for the Rapid Detection and Differentiation of the Most Relevant Members of the B. pseudomallei Complex: B. mallei, B. pseudomallei, and B. thailandensisSemantic proximity Read ↗

Development and validation of Burkholderia pseudomallei-specific real-time PCR assays for clinical, environmental or forensic detection applications.Semantic proximity Read ↗

Preliminary Evaluation of Rapid Visual Identification of Burkholderia pseudomallei Using a Newly Developed Lateral Flow Strip-Based Recombinase Polymerase Amplification (LF-RPA) SystemSemantic proximity Read ↗

Melioidosis molecular diagnostics: An updateSemantic proximity Read ↗

Development and Evaluation of a Real-Time PCR Assay Targeting the Type III Secretion System of <i>Burkholderia pseudomallei</i>Semantic proximity Read ↗

Rapid detection of Burkholderia pseudomallei with a lateral flow recombinase polymerase amplification assaySemantic proximity Read ↗

Laboratory diagnosis of melioidosis: Past, present and futureSemantic proximity Read ↗

PCR-based Methodologies Used to Detect and Differentiate the Burkholderia pseudomallei complex: B. pseudomallei, B. mallei, and B. thailandensis.Semantic proximity Read ↗

Highly Sensitive Direct Detection and Quantification of Burkholderia pseudomallei Bacteria in Environmental Soil Samples by Using Real-Time PCRSemantic proximity Read ↗

Novel Pan-Genomic Analysis Approach in Target Selection for Multiplex PCR Identification and Detection of Burkholderia pseudomallei, Burkholderia thailandensis, and Burkholderia cepacia Complex Species: a Proof-of-Concept StudySemantic proximity Read ↗

Comparison of TaqMan PCR Assays for Detection of the Melioidosis Agent Burkholderia pseudomallei in Clinical SpecimensSemantic proximity Read ↗

Loop-Mediated Isothermal Amplification Method Targeting the TTS1 Gene Cluster for Detection of <i>Burkholderia pseudomallei</i> and Diagnosis of MelioidosisSemantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.