Article
Development and validation of Burkholderia pseudomallei-specific real-time PCR assays for clinical, environmental or forensic detection applications.
PloS one - 1 Jan 2012
Price Erin P, Dale Julia L, Cook James M, Sarovich Derek S, Seymour Meagan L, Ginther Jennifer L, Kaufman Emily L, Beckstrom-Sternberg Stephen M, Mayo Mark, Kaestli Mirjam, Glass Mindy B, Gee Jay E, Wuthiekanun Vanaporn, Warner Jeffrey M, Baker Anthony, Foster Jeffrey T, Tan Patrick, Tuanyok Apichai, Limmathurotsakul Direk, Peacock Sharon J, Currie Bart J, Wagner David M, Keim Paul, Pearson Talima
Abstract excerpt
The bacterium Burkholderia pseudomallei causes melioidosis, a rare but serious illness that can be fatal if untreated or misdiagnosed. Species-specific PCR assays provide a technically simple method for differentiating B. pseudomallei from near-neighbor species. However, substantial genetic diversity and high levels of recombination within this species reduce the likelihood that molecular signatures will...
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