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Development and Evaluation of a Real-Time PCR Assay Targeting the Type III Secretion System of <i>Burkholderia pseudomallei</i>

1 Jan 2006

Abstract excerpt

Here we report on the development of a discriminatory real-time assay for the rapid identification of Burkholderia pseudomallei isolates and the evaluation of this assay for sensitivity against related species and detection in spiked human blood samples. The assay targets a 115-base-pair region within orf2 of the B. pseudomallei type III secretion system gene cluster and distinguishes B. pseudomallei from other...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Identifiers

PMID: 16390953
DOI: 10.1128/jcm.44.1.85-90.2006

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