Article
Inoculum cell count influences separation efficiency and variance in Ames plate incorporation and Ames RAMOS test.
The Science of the total environment - 20 Dec 2023
Forsten Eva, Finger Maurice, Scholand Theresa, Deitert Alexander, Kauffmann Kira, Büchs Jochen
Abstract excerpt
The Ames test is one of the most applied tools in mutagenicity testing of chemicals ever since its introduction by Ames et al. in the 1970s. Its principle is based on histidine auxotrophic bacteria that regain prototrophy through reverse mutations. In the presence of a mutagen, more reverse mutations occur that become visible as increased bacterial growth on medium without histidine. Many miniaturized formats of...
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