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Targeted sequencing analysis pipeline for species identification of human pathogenic fungi using long-read nanopore sequencing

6 Sept 2023

Abstract excerpt

Among molecular-based techniques for fungal identification, Sanger sequencing of the primary universal fungal DNA barcode, the internal transcribed spacer (ITS) region (ITS1, 5.8S, ITS2), is commonly used in clinical routine laboratories due to its simplicity, universality, efficacy, and affordability for fungal species identification. However, Sanger sequencing fails to identify mixed ITS sequences in the case...

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PMID: 37674240
DOI: 10.1186/s43008-023-00125-6

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