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Article

Replacement of the native cis prolines by alanine leads to simplification of the complex folding mechanism of a small globular protein.

Biophysical journal - 3 Oct 2023

Kaushik Anushka, Udgaonkar Jayant B

Abstract excerpt

The folding mechanism of MNEI, a single-chain variant of naturally occurring double-chain monellin, is complex, with multiple parallel refolding channels. To determine whether its folding energy landscape could be simplified, the two native cis-prolines, Pro41 and Pro93, were mutated, singly and together, to Ala. The stability of P93A was the same as that of the wild-type protein, pWT; however, P41A and P41AP93A...

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Identifiers

PMID: 37596784
DOI: 10.1016/j.bpj.2023.08.012
PMCID: PMC10560683

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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