The use of alkaline elution procedures to measure DNA damage in spermiogenic stages of mice exposed to methyl methanesulfonate.

doi:10.1016/0027-5107(86)90112-0

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Article

The use of alkaline elution procedures to measure DNA damage in spermiogenic stages of mice exposed to methyl methanesulfonate.

Mutation research - 1 Jan 2000

Sega G A, Sluder A E, McCoy L S, Owens J G, Generoso E E

Abstract excerpt

The ability of methyl methanesulfonate (MMS) to induce DNA breakage in spermiogenic stages of the mouse was studied using an alkaline elution technique. At daily intervals over a 3-week period following i.p. injection of 50 mg MMS/kg, mature spermatozoa were recovered from treated (3H-labeled) an...

Topics

Animals
DNA
Dose-Response Relationship, Drug
Hydrogen-Ion Concentration
Male
Methyl Methanesulfonate
Mice
Mutagenicity Tests
Mutation
Solubility
Spermatogenesis
Spermatozoa
Time Factors

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