Article
A pilot-scale comparison between single and double-digest RAD markers generated using GBS strategy in sesame (Sesamum indicum L.).
PloS one - 1 Jan 2023
Ruperao Pradeep, Bajaj Prasad, Subramani Rajkumar, Yadav Rashmi, Reddy Lachagari Vijaya Bhaskar, Lekkala Sivarama Prasad, Rathore Abhishek, Archak Sunil, Angadi Ulavappa B, Singh Rakesh, Singh Kuldeep, Mayes Sean, Rangan Parimalan
Abstract excerpt
To reduce the genome sequence representation, restriction site-associated DNA sequencing (RAD-seq) protocols is being widely used either with single-digest or double-digest methods. In this study, we genotyped the sesame population (48 sample size) in a pilot scale to compare single and double-digest RAD-seq (sd and ddRAD-seq) methods. We analysed the resulting short-read data generated from both protocols and...
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