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Article

Deep sampling and pooled amplicon sequencing reveals hidden genic variation in heterogeneous rye accessions.

BMC genomics - 30 Nov 2020

Hawliczek Anna, Bolibok Leszek, Tofil Katarzyna, Borzęcka Ewa, Jankowicz-Cieślak Joanna, Gawroński Piotr, Kral Adam, Till Bradley J, Bolibok-Brągoszewska Hanna

Abstract excerpt

BACKGROUND: Loss of genetic variation negatively impacts breeding efforts and food security. Genebanks house over 7 million accessions representing vast allelic diversity that is a resource for sustainable breeding. Discovery of DNA variations is an important step in the efficient use of these resources. While technologies have improved and costs dropped, it remains impractical to consider resequencing millions...

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PMID: 33256606
DOI: 10.1186/s12864-020-07240-3
PMCID: PMC7706248

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