Article
No Inhibitory Effect of Heparinized Blood on Real-Time PCR Analysis of Thrombophilic Mutations.
Folia biologica - 1 Jan 2022
Beránek M, Hegerová J, Michajlíková M, Dulíček P, Palička V
Abstract excerpt
We compared the efficiency of real-time PCR analysis of FII (c.*97G>A, G20210A) and FV Leiden (c.1601G>A) thrombophilic mutations in the samples obtained from venous blood treated with various anti coagulant agents (EDTA, heparin, and sodium fluoride with potassium oxalate), or from clotted venous blood; one hundred samples of wild-type subjects were tested. Genomic DNA extracts and whole blood specimens modified...
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