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Point-of-care detection of Neisseria gonorrhoeae based on RPA-CRISPR/Cas12a

27 May 2023

Abstract excerpt

Gonorrhea, caused by Neisseria gonorrhoeae (N. gonorrhoeae), is a persistent global public health threat. The development of low-cost, point-of-care testing is crucial for gonorrhea control, especially in regions with limited medical facilities. In this study, we integrated CRISPR/Cas12a reaction with recombinase polymerase amplification (RPA) to provide a simple and adaptable molecular detection method for N....

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PMID: 37243708
DOI: 10.1186/s13568-023-01554-7

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