Article
Develop a Compact RNA Base Editor by Fusing ADAR with Engineered EcCas6e.
Advanced science (Weinheim, Baden-Wurttemberg, Germany) - 1 Jun 2023
Wang Xing, Zhang Renxia, Yang Dong, Li Guoling, Fan Zhanqing, Du Hongting, Wang Zikang, Liu Yuanhua, Lin Jiajia, Wu Xiaoqing, Shi Linyu, Yang Hui, Zhou Yingsi
Abstract excerpt
Catalytically inactive CRISPR-Cas13 (dCas13)-based base editors can achieve the conversion of adenine-to-inosine (A-to-I) or cytidine-to-uridine (C-to-U) at the RNA level, however, the large size of dCas13 protein limits its in vivo applications. Here, a compact and efficient RNA base editor (ceRBE) is reported with high in vivo editing efficiency. The larger dCas13 protein is replaced with a 199-amino acid...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.