Article
Impact of G29179T mutation on two commercial PCR assays for SARS-CoV-2 detection.
Journal of virological methods - 1 Apr 2023
Kami Wakaki, Kinjo Takeshi, Hashioka Hiroe, Arakaki Wakako, Uechi Kohei, Takahashi Ami, Oki Hiroya, Tanaka Kentaro, Motooka Daisuke, Nakamura Shota, Nakamatsu Masashi, Maeda Shiro, Yamamoto Kazuko, Fujita Jiro
Abstract excerpt
Nucleic acid amplification test (NAAT) is the gold standard for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) detection. However, genetic mutations in the virus can affect the result. Cycle threshold (Ct) values of N genes and their association with mutations using SARS-CoV-2 positive specimens diagnosed by the Xpert Xpress SARS-CoV-2 were examined in this study. In total, 196 nasopharyngeal swab...
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