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Article

2b-RAD: a simple and flexible method for genome-wide genotyping.

Nature methods - 20 May 2012

Wang Shi, Meyer Eli, McKay John K, Matz Mikhail V

Abstract excerpt

We describe 2b-RAD, a streamlined restriction site-associated DNA (RAD) genotyping method based on sequencing the uniform fragments produced by type IIB restriction endonucleases. Well-studied accessions of Arabidopsis thaliana were genotyped to validate the method's accuracy and to demonstrate fine-tuning of marker density as needed. The simplicity of the 2b-RAD protocol makes it particularly suitable for...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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PMID: 22609625
DOI: 10.1038/nmeth.2023

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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