Article
Design and characterization of a protein fold switching network.
Nature communications - 26 Jan 2023
Ruan Biao, He Yanan, Chen Yingwei, Choi Eun Jung, Chen Yihong, Motabar Dana, Solomon Tsega, Simmerman Richard, Kauffman Thomas, Gallagher D Travis, Orban John, Bryan Philip N
Abstract excerpt
To better understand how amino acid sequence encodes protein structure, we engineered mutational pathways that connect three common folds (3α, β-grasp, and α/β-plait). The structures of proteins at high sequence-identity intersections in the pathways (nodes) were determined using NMR spectroscopy and analyzed for stability and function. To generate nodes, the amino acid sequence encoding a smaller fold is...
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