Article
ABC-transporter CFTR folds with high fidelity through a modular, stepwise pathway.
Cellular and molecular life sciences : CMLS - 7 Jan 2023
Im Jisu, Hillenaar Tamara, Yeoh Hui Ying, Sahasrabudhe Priyanka, Mijnders Marjolein, van Willigen Marcel, Hagos Azib, de Mattos Eduardo, van der Sluijs Peter, Braakman Ineke
Abstract excerpt
The question how proteins fold is especially pointed for large multi-domain, multi-spanning membrane proteins with complex topologies. We have uncovered the sequence of events that encompass proper folding of the ABC transporter CFTR in live cells by combining kinetic radiolabeling with protease-susceptibility assays. We found that CFTR folds in two clearly distinct stages. The first, co-translational, stage...
Topics
- Humans
- Cystic Fibrosis Transmembrane Conductance Regulator
- Protein Structure, Tertiary
- Cystic Fibrosis
- Mutation
- Peptide Hydrolases
- Protein Folding