Article
Room temperature crystal structure of the fast switching M159T mutant of the fluorescent protein dronpa.
Proteins - 1 Mar 2015
Kaucikas Marius, Fitzpatrick Ann, Bryan Elana, Struve Abelone, Henning Robert, Kosheleva Irina, Srajer Vukica, Groenhof Gerrit, Van Thor Jasper J
Abstract excerpt
The fluorescent protein Dronpa undergoes reversible photoswitching reactions between the bright "on" and dark "off" states via photoisomerization and proton transfer reactions. We report the room temperature crystal structure of the fast switching Met159Thr mutant of Dronpa at 2.0-Å resolution in...
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