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Article

Cyclic Multiplex Fluorescent Immunohistochemistry Protocol to Phenotype Glial Cells in Formalin-Fixed Paraffin-Embedded Human Brain Sections.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2023

Muñoz-Castro Clara, Noori Ayush, Hyman Bradley T, Serrano-Pozo Alberto

Abstract excerpt

There is a growing interest in expanding the multiplexing capability of immunohistochemistry to achieve a deeper phenotyping of various cell types in health and disease. Here, we describe a protocol of cyclic multiplex fluorescent immunohistochemistry that enables the labeling of up to 16 antigens on the same formalin-fixed paraffin-embedded section using "off-the-shelf," commercially available, primary...

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Identifiers

PMID: 36513939
DOI: 10.1007/978-1-0716-2811-9_19
PMCID: PMC9900949

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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